Journal
ELECTROPHORESIS
Volume 36, Issue 3, Pages 471-474Publisher
WILEY-BLACKWELL
DOI: 10.1002/elps.201400284
Keywords
AC electrothermal effect; Capacitive affinity sensing dielectrophoresis; Immunosensor
Funding
- U.S. National Science Foundation [ECS-0448896]
- China Natural Science Foundation [11372093, 51305106]
- University of Tennessee Research Foundation Maturation Fund
- M-CERV seed grant
- AgResearch Innovation Grant
- Scientific User Facilities Division, Office of Basic Energy Sciences, U.S. Department of Energy
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A rapid in situ capacitive immunoassay is presented herein. Conventional immunoassay typically relies on diffusion for transport of analytes in many cases causing long detection time and lack of sensitivity. By integrating alternating current electrokinetics (ACEK) and impedance sensing, this work provides a rapid in situ capacitive affinity biosensing. ACEK induces both fluid flow and particle motion, conveying target molecules toward electrodes immobilized with probes, resulting in rapid enrichment of target molecules and a capacitance change at the electrode-fluid'' interface. The benefit of ACEK enhanced immunoassay was demonstrated using the antigen and antibody from Johne's disease (JD) as an example. To clarify the importance of DEP and ACET effects for binding reaction, two different electrode pattern designs for capacitive immunoassay are studied. The asymmetric array and symmetric electrodes exhibit very similar response at lower electric field due to DEP effects, while asymmetric array has remarkable higher response at high-electric field because the convection becomes more important at high field. The disease positive and negative serum samples are distinguished in few minutes.
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