Journal
ELECTROPHORESIS
Volume 33, Issue 8, Pages 1251-1254Publisher
WILEY
DOI: 10.1002/elps.201100556
Keywords
Background staining; Gel electrophoresis; Gold nanoparticles
Funding
- Graz University of Technology
- Syracuse University
- National Science Foundation [OISE-0456116]
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Gel electrophoresis is a powerful tool in gold nanoparticle (AuNP) research. While the technique is sensitive to the size, charge, and shape of particles, its optimal performance requires a relatively large amount of AuNP in the loading wells for visible detection of bands. We here describe a novel and more sensitive method for detecting AuNPs in agarose gels that involves staining the gel with the common organic fluorophore fluorescein, to produce AuNP band intensities that are linear with nanoparticle concentration and almost an order of magnitude larger than those obtained without staining the gel.
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