Journal
ELECTROPHORESIS
Volume 32, Issue 14, Pages 1804-1806Publisher
WILEY-BLACKWELL
DOI: 10.1002/elps.201100118
Keywords
Bovine skeletal muscle; Myosin heavy chain isoform; SDS-PAGE
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In this short communication we describe a specific protocol for SDS-PAGE separation of adult bovine myosin heavy-chain (MyHC) isoforms. The conditions defined in this protocol allow a good separation with a good reproducibility of the four MyHC isoforms (MyHC I, IIa, IIx, IIb) identified in adult skeletal muscle of this species. This procedure uses mini-gel electrophoresis system and does not involve preparation of gradient separating gels. In addition, this protocol can also be applied to the electrophoretic separation of ovine and camel MyHC isoforms.
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