Journal
ELECTROPHORESIS
Volume 32, Issue 16, Pages 2099-2106Publisher
WILEY-BLACKWELL
DOI: 10.1002/elps.201100215
Keywords
CZE; Fe(3)O(4); SiO(2); poly(MAA-co-EDMA); magnetic microspheres; Illegal drugs; Magnetic solid-phase extraction
Funding
- National Science Fund for Distinguished Young Scholars [20625516]
- National Nature Science Foundation [91017013, 31070327]
- Science Fund for Creative Research Groups [20921062]
- NSFC
- National 973 project of China [2007CB914200]
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Using magnetite/silica/poly(methacrylic acid-co-ethylene glycol dimethacrylate) (Fe(3)O(4)/SiO(2)/poly(MAA-co-EDMA)) magnetic microspheres, a rapid and high-throughput magnetic solid-phase extraction coupled with capillary zone electrophoresis (MSPE-CZE) method was developed for the determination of illegal drugs (ketamine, amphetamines, opiates, and metabolites). The MSPE of target analytes could be completed within 2 min, and the eight target analytes could be baseline separated within 15 min by CZE with 30 mM phosphate buffer solution (PBS, pH 2.0) containing 15% v/v ACN as background electrolyte. Furthermore, hydrodynamic injection with field-amplified sample stacking (FASS) was employed to enhance the sensitivity of this MSPE-CZE method. Under such optimal conditions, the limits of detection for the eight target analytes ranged from 0.015 to 0.105 mu g/mL. The application feasibility of MSPE-CZE in illegal drugs monitoring was demonstrated by analyzing urine samples, and the recoveries of target drugs for the spiked sample ranging from 85.4 to 110.1%. The method reproducibility was tested by evaluating the intra- and interday precisions, and relative standard deviations of < 10.3 and 12.4%, respectively, were obtained. To increase throughput of the analysis, a home-made MSPE array that has potential application to the treatment of 96 samples simultaneously was used.
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