Journal
ELECTROPHORESIS
Volume 32, Issue 8, Pages 884-889Publisher
WILEY
DOI: 10.1002/elps.201000420
Keywords
CE; Contactless conductivity detection; Lactate; Lactate threshold; Plasma lactate
Funding
- Thai Government
- Swiss National Science Foundation [200020-126384/1]
- Swiss National Science Foundation (SNF) [200020_126384] Funding Source: Swiss National Science Foundation (SNF)
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The quantification of plasma lactate and evaluation of the lactate threshold by CE with capacitively coupled contactless conductivity is demonstrated. The only sample preparation needed was deproteinization with a ACN/methanol mixture. A solution of 10 mmol/L 2-morpholinoethanesulfonic acid monohydrate, 10 mmol/L DL-histidine, 70 mu mol/L hexadecyltrimethylammonium bromide, pH 6.0 was found suitable as running buffer. Linearity was achieved for the concentration range of 10-1000 mu mol/L with a correlation coefficient of 0.9994. The limit of detection (3 S/N) was determined as 3.2 mu mol/L. Intra-and inter-day variabilities were less than 7% RSD. The suitability of the method could be demonstrated by analyzing various clinical samples, where the results correlated satisfactorily with those of an established enzymatic method.
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