Journal
ELECTROPHORESIS
Volume 32, Issue 2, Pages 292-299Publisher
WILEY
DOI: 10.1002/elps.201000399
Keywords
Capillary electrophoresis; Cysteinylation; Degradation; Dimerization; Human serum albumin
Funding
- Albaath University of Syria
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We present a new CZE method, which uses a polyethylene oxide-coated capillary to separate native HSA from more than five of its structural variants. These variants include oxidized, truncated, and cysteinylated forms of HSA which can all be found in biopharmaceutical products. Both CE and MS confirmed the high degree of heterogeneity of HSA preparations. Recovery studies demonstrated that adsorption of HSA on the capillary was significantly reduced under the conditions we developed, which led to a satisfactory repeatability (RSD for migration times and relative peak areas were less than 0.2 and 7.0%, respectively). Assignment of the main peaks was attempted using in vitro degraded/stressed HSA. We used our method to test batch-to-batch comparability and detected slight quantitative differences in the proportion of native HSA in batches produced from different fractionation methods.
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