4.5 Review

Recent developments and applications of EMMA in enzymatic and derivatization reactions

Journal

ELECTROPHORESIS
Volume 33, Issue 1, Pages 211-227

Publisher

WILEY-BLACKWELL
DOI: 10.1002/elps.201100366

Keywords

CE; Derivatization; Electrophoretically mediated microanalysis; Enzyme; In-capillary reaction

Funding

  1. Flemish Fund for Scientific Research [G.0681.08]

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This review provides systematic coverage of examples in the field of in-capillary electrophorecially mediated microanalysis (EMMA). The recent developments and applications in the time period up to mid 2011 have been described, as well as relevant older papers. The basic principles and modes of in-capillary assays have been demonstrated. An overview is also given of the various injection, separation and detection modes implemented in combination with EMMA. The review is presented in two parts mainly dealing with (i) enzymatic and (ii) derivatization or chemical reactions. Finally, the future trends of CE in performing and monitoring reactions have been drawn.

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