Journal
ELECTROPHORESIS
Volume 32, Issue 21, Pages 3034-3041Publisher
WILEY
DOI: 10.1002/elps.201100216
Keywords
alpha-Hemolysin; Acidic pH; Nanopore; Single-stranded DNA; Translocation
Funding
- National Institutes of Health [1R011HG005095]
Ask authors/readers for more resources
The effect of acidic pH on the translocation of single-stranded DNA through the alpha-hemolysin pore is investigated. Two significantly different types of events, i.e. deep blockades and shallow blockades, are observed at low pH. The residence times of the shallow blockades are not significantly different from those of the DNA translocation events obtained at or near physiological pH, whereas the deep blockades have much larger residence times and blockage amplitudes. With a decrease in the pH of the electrolyte solution, the percentage of the deep blockades in the total events increases. Furthermore, the mean residence time of these long-lived events is dependent on the length of DNA, and also varies with the nucleotide base, suggesting that they are appropriate for use in DNA analysis. In addition to being used as an effective approach to affect DNA translocation in the nanopore, manipulation of the pH of the electrolyte solution provides a potential means to greatly enhance the sensitivity of nanopore stochastic sensing.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available