Journal
ELECTROPHORESIS
Volume 31, Issue 23-24, Pages 3928-3936Publisher
WILEY
DOI: 10.1002/elps.201000352
Keywords
Biacetyl; Chiral separation; Electrokinetic chromatography; Energy transfer
Funding
- Ministry of Science and Innovation (Spain) (Movilidad RRHH del Plan Nacional I-D+I) [2008-0288]
- Dutch Foundation for Advancement of Science (NWO-CW) [700.55.014]
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A new CE detection method was developed for the chiral drug bupropion (a second-generation antidepressant), based on phosphorescence both in the direct and in the sensitized mode using pulsed laser excitation at 266 nm. Electrokinetic chromatography using 5 mM sulfated-alpha-CD as chiral selector in 25 mM phosphate buffer at pH 3 allowed the separation of bupropion enantiomers with a high chiral resolution (Rs > 3). In the sensitized phosphorescence detection mode, excitation energy is transferred from the analyte to an acceptor (1-bromo-4-napthhalenesulfonic acid or biacetyl) followed by time-resolved phosphorescence detection under deoxygenated buffer conditions. Using 2 x 10(-4) M biacetyl as the acceptor an LOD of 2 x 10(-7) M was obtained for each enantiomer, about 40 times better than in the direct mode. Under these separation conditions, no significantly different phosphorescence lifetimes (measured on-line) were obtained for the two bupropion enantiomers. The suitability of the method was demonstrated with the quantification of bupropion in a pharmaceutical formulation and its determination in a spiked urine sample.
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