Journal
ELECTROPHORESIS
Volume 31, Issue 12, Pages 2044-2048Publisher
WILEY
DOI: 10.1002/elps.201000127
Keywords
Amino acids; CE; Contactless conductivity detection; Enantiomeric separation; Hydroxycarboxylic acids
Funding
- Swiss National Science Foundation [200020-113335/1, PMCD2-114322/1]
- Thai Government
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The enantiomers of the anions of five alpha-hydroxy acids, namely lactic acid, alpha-hydroxybutyric acid, 2-hydroxycaproic acid, 2-hydroxyoctanoic acid and 2-hydroxydecanoic acid, as well as the two alpha-amino acids aspartic acid and glutamic acid, were baseline separated and detected by CE with contactless conductivity detection. Vancomycin was employed as chiral selector and could be used with conductivity detection without having to resort to a partial filling protocol as needed when this reagent is used with UV absorbance measurements. The procedure was successfully applied to the determination of the lactic acid enantiomers in samples of milk and yogurt. Linearity was achieved in the concentration range of 10-500 mu mol/L with good correlation coefficients (0.9993 and 0.9990 for L- and D-lactic acid, respectively). The LODs (3 S/N) for L- and D-lactic acid were determined as 2.8 and 2.4 mu mol/L, respectively.
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