Journal
ELECTROPHORESIS
Volume 31, Issue 16, Pages 2822-2830Publisher
WILEY
DOI: 10.1002/elps.201000035
Keywords
CEC; Molecularly imprinted polymer; Monolithic column; Nitroimidazole drugs
Funding
- National Natural Science Foundation [20907009, 40976071, 20905079]
- Program for New Century Excellent Talents in University of China [NECT-06-0572]
- Program for New Century Excellent Talents in University of Fujian Province [HX2006-99]
- Key Science & Technology Project of Fujian Province [2008Y2004, 2008Y0020, 2006BAK02A21-1-2]
- Natural Science Foundation of Fujian Province [2009J0102, 2007J0138]
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A polymethacrylate-based molecularly imprinted monolithic column bearing mixed functional monomers, using non-covalent imprinting approach, was designed for the rapid separation of nitroimidazole compounds. The new monolithic column has been prepared via simple in situ polymerization of 2-hydroxyethyl methacrylate, dimethylaminoethyl methacrylate and ethylene dimethacrylate, using (S)-ornidazole ((S)-ONZ) as template in a binary porogenic mixture consisting of toluene and dodecanol. The composition of the polymerization mixture was systematically altered and optimized by altering the amount of monomers as well as the composition of the porogenic solvent. The column performance was evaluated in pressure-assisted CEC mode. Separation conditions such as pH, voltage, amount of organic modifier and salt concentration were studied. The optimized monolithic column resulted in excellent separation of a group of structurally related nitroimidazole drugs within 10 min in isocratic elution condition. Column efficiencies of 99 000, 80000, 103 000, 60 000 and 99 000 plates/m were obtained for metronidazole, secnidazole, ronidazole, tinidazole and dimetridazole, respectively. Parallel experiments were carried out using molecularly imprinted and non-imprinted capillary columns. The separation might be the result of combined effects including hydrophobic, hydrogen bonding and the imprinting cavities on the (S)-ONZ-imprinted monolithic column.
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