Journal
ELECTROPHORESIS
Volume 30, Issue 23, Pages 4149-4151Publisher
WILEY
DOI: 10.1002/elps.200900474
Keywords
Anaerobic digestion; Metaproteomics; Sludge granules; Wastewater treatment
Funding
- Science Foundation Ireland
Ask authors/readers for more resources
The feasibility of metaproteomic analysis of the microbial communities underpinning anaerobic wastewater treatment relies on efficient protein extraction and separation techniques. The microorganisms involved in anaerobic digestion (AD) of wastewater typically aggregate to form tightly organised, spherical granules, from which protein extraction is challenging. Here, we compare two methods of protein extraction, one using a French press previously used successfully to analyse the proteome of an activated sludge [Wilmes, P., Bond, P. L., Environ. Microbiol. 2004, 6, 911-920] and one using sonication developed in the context of pure culture [Abram, F., Wan-Lin, S., Wiedmann, M, Boor, K J., Coote, P., Botting, C., Karatzas, K. A. G., O'Byrne, C. P, Appl. Environ. Microbiol. 2008, 74, 594-604.]. We used 2-DE to carry out this comparison. The protein extraction using the sonication method resulted in a significant increase in the number of protein spots and higher quality 2-D gels.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available