Journal
ELECTROPHORESIS
Volume 30, Issue 19, Pages 3436-3441Publisher
WILEY
DOI: 10.1002/elps.200900187
Keywords
Amino acids; Contactless conductivity detection; Neurotransmitters; Pain; Stacking
Funding
- Grant Agency of the Czech Republic [20310710896]
- Ministry Of Education, Youth and Sports, Czech Republic [MSM0021620814, MSM0021620816]
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CE with contactless conductivity detection has been used to determine the glycine neurotransmitter in periaqueductal gray matter (PAG) of rats. The LOD for glycine has been decreased to a value of 0.2 mu M by adding 75% v/v of ACN to the samples and increasing the sample zone introduced to a value of 20% of the overall capillary length. The repeatabilities of the analyte migration times and the zone areas amount to 2.1 and 2.7%, respectively. The optimized CE/contactless conductivity detection method makes it possible to determine the micromolar concentrations of glycine in PAG microdialyzates without the necessity of sample derivatization. It follows from a pharmacological study that a local inflammation initiated by an application of carrageenan increased the glycine concentration in the rat PAG seven times, compared with a control. The glycine level in PAG can be decreased and the pain suppressed by administering paracetamol.
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