Journal
ELECTROPHORESIS
Volume 30, Issue 3, Pages 499-506Publisher
WILEY
DOI: 10.1002/elps.200800305
Keywords
Amperometric detection; CE; Macrocyclic lactone mycotoxins screening; Maize flour samples; Supercritical fluid extraction
Funding
- Ministerio de Educacion y Ciencia of Spain [CTQ2004-06334-C02-01-02, CTQ2007-61830]
- JJCC Castilla-La Mancha [PCC08-015-0722]
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A simple analytical scheme for the screening and quantification of zearalenone and its metabolites, alpha-zearalenol and P-zearalenol, is reported. Extracts from maize flour samples were collected by supercritical fluid extraction and afterwards, they were analyzed by CE with amperometric detection. This scheme allowed a rapid and reliable identification of contaminated flour samples according to the reference value established for zearalenone by directive 2005/38/EC (200 mu g/kg). The sample screening method was carried out by CZE using 25 mM borate separation buffer at PH 9.2 and 25.0 kV as separation voltage, monitoring the amperometric signal at + 700 mV with a carbon paste electrode. In this way, total amount of mycotoxins was determined and samples were processed in 4 min with a detection limit of 12 mu g/L, enough to discriminate between positive (more than 200 mu g/L total mycotoxins) and negative samples (less than 200 mu g/L total mycotoxins). Positive samples were then subjected to CZE separation and quantification of each analyte was done with 50 mM borate running buffer modified with 30% methanol at pH 9.7 and 17.5 kV as separation voltage. Under these conditions, separation was achieved in 15 min with detection limits from 20 to 35 mu g/L for each analyte.
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