Journal
ELECTROPHORESIS
Volume 30, Issue 18, Pages 3250-3256Publisher
WILEY-BLACKWELL
DOI: 10.1002/elps.200900111
Keywords
BSA; Immunoassay; ITP concentration; PMMA microchip
Funding
- Ministry of Education, Culture, Sports, Science and Technology, Japan
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In this research, a simple on-line microchip gel electrophoresis with ITP was applied for the concentration and separation of BSA and its immunoassay complex with mAb in a single cross form PMMA microchip. We investigated the ITP concentration effect in PMMA MCE using combination of leading electrolytes, terminating electrolytes and other factors. We realized an ITP-based concentration and separation of BSA and its immunoassay complexes in standard cross-channel microchip gel electrophoresis, which exceeded 2000-fold concentration of BSA immunocomplex using Tris-H3PO4 as a leading electrolyte and Tris-gamma-amino butyric acid as a terminating electrolyte. in addition, we also realized concentration of BSA sample in water, which was more than 20 000-fold and was the result of the concentration effect from combining ITP and the sample stacking techniques.
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