Journal
ELECTROPHORESIS
Volume 30, Issue -, Pages S40-S52Publisher
WILEY
DOI: 10.1002/elps.200900156
Keywords
Biomolecules; Bioparticles; CE; Free-flow electrophoresis; Preparative separation
Funding
- Agency of the Czech Republic [203/96/K128, 203/05/2539, 203/08/1428]
- Academy of Sciences of the Czech Republic [Z40550506]
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This invited contribution in the special issue of Electrophoresis published in celebration of the 30th Anniversary of this journal reflects the impact of our milestone paper [Prusik, Z., Kasicka, V., Mudra, P., Stepanek, J., Smekal, O., Hlavacek, J., Electrophoresis 1990, 11, 932-936] in the area of conversion of microscale analytical and micropreparative CE separations of biomolecules and bioparticles into (macro) preparative freeflow electrophoresis (FFE) scale on the basis of a correlation between CE and FFE methods. In addition to the survey of advances in the relatively narrow field of CE-FFE correlation and CE-FFE conversion, a comprehensive review of the recent developments of micropreparative CE and (macro) preparative FFE techniques is also presented and applications of these techniques to micro- and (macro) preparative separations and purifications of biomolecules and bioparticles are demonstrated. The review covers the period since the year of publication of the above paper, i.e. ca. the last 20 years.
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