Journal
ELECTROPHORESIS
Volume 30, Issue 23, Pages 4008-4019Publisher
WILEY
DOI: 10.1002/elps.200900211
Keywords
Clinical proteomics/diagnostics; Microsphere-based flow cytometric platforms; Multiplexed assay; Suspension microarray
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Advances in high-throughput screening, together with rapid progress in genomic and proteomic sciences, have considerably stimulated the development of a variety of biomarker discovery tools and have contributed to the improvement of clinical diagnosis Major challenges include the obtaining of high-quality data sets based on assays that are rapid, reliable, and inexpensive, but still highly sensitive and easy to perform. The microsphere-based flow cytometric technology is a platform that is currently entering the diagnostic market. This article reviews the development and the applications of microsphere-based flow cytometric platforms in basic and applied proteomic research, and discusses bead-based assays used in clinical applications.
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