4.5 Article

On-chip tryptic digest with direct coupling to ESI-MS using magnetic particles

Journal

ELECTROPHORESIS
Volume 29, Issue 24, Pages 4944-4947

Publisher

WILEY
DOI: 10.1002/elps.200800431

Keywords

Magnetic beads; MS; Self-organization

Funding

  1. French Ministry of Research/CNRS Programme
  2. European Project
  3. IACH [Z40310501]
  4. Grant Agency of the Czech Republic [20310611685]
  5. Czech Academy of Sciences [KAN400310651]

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As a step toward a fully automated front-end microfluidic chip for MS proteomics, we propose a system capable of performing online tryptic digest and ESI-MS, using a replaceable on-chip digestion microcolumn based on self-assembled magnetic particles.

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