Journal
ELECTROPHORESIS
Volume 29, Issue 24, Pages 4900-4905Publisher
WILEY
DOI: 10.1002/elps.200800463
Keywords
Amino acids; IMEKC; Sodium lauryl ether sulfate
Funding
- NSF [CHE-0548046]
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A new buffer has been developed for fast, high-efficiency separations of amino acids by MEKC. This buffer was more environmentally friendly than the most commonly used surfactant-containing buffers for MEKC separations. It used a commercially available dishwashing soap by Seventh Generation (TM) (Burlington, VT, USA), which contained three micelle-forming agents. The mixed micelles were composed of sodium lauryl ether sulfate (anionic), cocamidopropyl betaine (zwitterionic), and cocamide mono-ethanolamine (non-ionic). The optimized buffer contained 5.0% w/w Seventh Generation (TM) Free & Clear (TM) dishwashing soap, 10 mM sodium borate, and was completely void of organics. The lack of organics and the biodegradability of the surfactant molecules made this buffer more environmentally friendly than typical SDS-containing buffers. This new buffer also had a different selectivity and provided faster separations with higher separation efficiencies than SDS-based buffers. Fast separations of BODIPY FL labeled amino acids yielded peaks with separation efficiencies greater than 100 000 in less than 20 s.
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