Journal
ELECTROPHORESIS
Volume 29, Issue 21, Pages 4381-4385Publisher
WILEY
DOI: 10.1002/elps.200800089
Keywords
Conformation; Phosphopeptide; Tau protein; Trifluoroethanol
Funding
- Deutsche Forschungsgemeinschaft
- European Fond for Regional Structure Development (EFRE, European Union and Free State Saxony)
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A separation of mono, doubly and triply phosphorylated isomers was developed with CZE with an aqueous electrolyte containing 3.9 mol/L formic acid and 30% v/v trifluoroethanol. Thus a mixture of ten phosphopeptides corresponding to the human tau sequence 226-240 was separated within 70 min. Although peptides with different phosphorylation degrees, i.e. 0-3 phosphate groups, were well separated, some of the phosphopeptide isomers containing one or two phosphate groups were only partially separated. The electrolyte system is compatible with both MALDI- and ESI-MS, allowing a direct coupling, and thus could have some interesting applications in proteomics.
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