Journal
ELECTROPHORESIS
Volume 29, Issue 8, Pages 1612-1618Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elps.200700513
Keywords
automation; monolithic analytical capillary column; nano-LC-MS/MS; peptide separation; sample introduction
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An automated nano-LC-MS/MS platform without trap column was established, which only used a 20 cm lauryl methacrylate-ethylene dimethacrylate (LMA-EDMA) monolithic capillary column to allow preconcentration and separation of peptides. The monolithic column had the advantages of good permeability and low backpressure resulting in higher flow rates for capillary columns. Tryptic digests of bovine albumin and yeast protein extract were tested using the monolithic column system. High proteomic coverage using this approach were demonstrated in this study. Furthermore, peptide samples extracted from mouse liver were separated by using the monolithic column system combined with size-exclusion chromatography prefractionation. This monolithic column system might be a promising alternative for the automated system previously using a trap column for routine proteome and peptide profiling analysis.
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