Journal
ELECTROPHORESIS
Volume 29, Issue 24, Pages 4928-4937Publisher
WILEY
DOI: 10.1002/elps.200700897
Keywords
EOF; Microchip electrophoresis; Separation efficiency; Substrate material
Funding
- Coordenadoria de Aperfeicoamento de Pessoal de Ensino Superior (CAPES) [BEX0706/02-1]
- National Institutes of Health [NS049229]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [04/01525-0]
- Conselho Nacional de Desenvolvimento Cientifico Tecnologico (CNPq) [478467/2006-0]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [04/01525-0] Funding Source: FAPESP
Ask authors/readers for more resources
This paper compares the analytical performance of microchannels fabricated in PDMS, glass, and polyester-toner for electrophoretic separations. Glass and PDMS chips were fabricated using well-established photolithographic and replica-molding procedures, respectively. PDMS channels were sealed against three different types of materials: native PDMS, plasma-oxidized PDMS, and glass. Polyester-toner chips were micromachined by a direct-printing process using an office laser printer. All microchannels were fabricated with similar dimensions according to the limitations of the direct-printing process (width/depth 150 mu m/12 mu m). LIF was employed for detection to rule out any losses in separation efficiency due to the detector configuration. Two fluorescent dyes, coumarin and fluorescein, were used as model analytes. Devices were evaluated for the following parameters related to electrophoretic separations: EOF, heat dissipation, injection reproducibility, separation efficiency, and adsorption to channel wall.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available