Simultaneous determination of dopamine and uric acid in the presence of ascorbic acid using Pt nanoparticles supported on reduced graphene oxide

In this paper, a Pt/reduced graphene oxide (Pt/RGO) modified glassy carbon electrode was prepared for thedetection of dopamine (DA) and uric acid (UA) in the presence of high concentration of ascorbic acid (AA). The electrochemical behavior of the Pt/RGO modified electrode was characterized by cyclic voltammetry and differential pulse voltammetry, which showed good performance toward individual detection of DA and UA and even their simultaneous detection in the presence of 1.0 mM AA. Evidently, the electro-oxidation peak currents displayed linear relationship with the associated DA and UA concentrations in the range of 10.0-170.0 mu M and 10.0-130.0 mu M, respectively, with the detection limits of 0.25 mu M for DA and 0.45 mu M for UA at three folds of the signal-to-noise ratio. The good performance of the Pt/RGO modified electrode provided a promising alternative in routine sensing applications. (C) 2013 Elsevier Ltd. All rights reserved.