Journal
ELECTROCHIMICA ACTA
Volume 67, Issue -, Pages 140-146Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.electacta.2012.02.011
Keywords
Glucose oxidase (GOx); Electron transfer; Bioelectrochemical sensor; Gold film; Electrochemical detection
Categories
Funding
- National Natural Science Foundation of China [21073111, 21175059]
- National Basic Research Program of China [2009CB930103]
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Glucose oxidase (GOx) was stably immobilized via a simple physical adsorption method onto the nanostructured Au thin films fabricated by using electrodeposition and galvanic replacement technology, which provides a facile method to prepare morphology-controllable Au films and also facilitates the preparation and application of enzyme modified electrodes. An obvious advantage of the as-prepared enzyme electrode (denoted as GOx/Au/GCE) is that the nano-Au films provide a favorable microenvironment for GOx and facilitate the electron transfer between the active center of GOx and electrodes. Cyclic voltammetry (CV) results indicate that the immobilized GOx displayed a direct, reversible and surface-confined redox reaction in the phosphate buffer solution. Furthermore, the enzyme modified electrode was used as a glucose bioelectrochemical sensor, exhibiting a linear relationship in the concentration ranges of 2.5-32.5 mu mol L-1 and 60-130 mu mol L-1 with a detection limit of 0.32 mu mol L-1 (S/N = 3) at an applied potential of -0.55 V. Due to the excellent stability, sensitivity and anti-interference ability, the Au thin films are hopeful in the construction of glucose biosensors. (C) 2012 Elsevier Ltd. All rights reserved.
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