Journal
ELECTROCHIMICA ACTA
Volume 56, Issue 20, Pages 6919-6924Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.electacta.2011.06.023
Keywords
Au nanoparticle; Interfacial assembly; Layer-by-layer assembly; Horseradish peroxidase; Biosensor
Categories
Funding
- NSFC [20703016]
- Scientific and Technological Projects of Hunan Province [2010FJ6030]
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Au nanoparticle (AuNP) multilayer films were fabricated by combining interfacial assembly and layer-by-layer assembly. The key point is that the procedure does not require assistance of organic linker molecules, thus providing a suitable platform for the modification of biological molecules. Direct electron transfer can easily take place between a glassy carbon electrode and horseradish peroxidase (HRP) molecules adsorbed on AuNP films. The current density of direct electron transfer was closely related to the layer number, m, and reached a maximum value for m = 4. The optimized HRP/AuNP multilayer film had a relatively rapid response and satisfactory selectivity for H2O2 detection. The linear range and the detection limit were 9.8 x 10(-6) to 6 x 10(-3) mol/L and similar to 4.9 x 10(-6) mol/L (S/N=3), respectively. (C) 2011 Elsevier Ltd. All rights reserved.
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