Journal
ELECTROCHIMICA ACTA
Volume 56, Issue 6, Pages 2725-2729Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.electacta.2010.12.051
Keywords
Single-base mismatches; DNA hybridization biosensor; Nanoparticle-functionalized nucleic acid; Thermodynamically stable mismatches
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Funding
- research council of the University of Isfahan
- Iranian Biotechnology Development Council
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In this study, nanoparticle-functionalized nucleic acids were employed to improve the sensitivity of electrochemical DNA biosensors that make capable them to detect different types of single-base mismatches (SBMs), including thermodynamically stable ones. The present biosensor was constructed by the immobilization of platinum nanoparticles (Pt-NPs) on the surface of a carbon paste electrode (CPE) via SH-functionalized DNA. A redox probe of 2-mercapto-1-methyl imidazole (MMI), which has different electrochemical behavior on Pt-NP and CPE, was used. This behavior helps to overcome the pinhole effect in DNA hybridization biosensors. Additionally, in the present biosensor, the positioning of the redox probe under the SBM in DNA, which decreases the sensitivity of most DNA biosensors, did not contribute to the observed electrochemical signal. (C) 2010 Elsevier Ltd. All rights reserved.
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