Journal
ELECTROCHIMICA ACTA
Volume 54, Issue 28, Pages 7330-7337Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.electacta.2009.07.060
Keywords
Layer-by-layer assembly; Biospecific recognition; Concanavalin A; Myoglobin; Direct electrochemistry
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Funding
- National Natural Science Foundation of China (NSFC) [20475008, 20775009]
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Lectin protein concanavalin A (Con A) and polysaccharide dextran (Dex) were assembled into {Con A/Dex} layer-by-layer (LBL) films by biospecific affinity between them. The films were then immersed in myoglobin (Mb) solution to load Mb into the films, designated as {Con A/Dex}(n)-Mb. Quartz crystal microbalance (QCM), cyclic voltammetry (CV), square wave voltammetry (SWV). and electrochemical impedance spectroscopy (EIS) were used to monitor the growth of {Con A/Dex}(n) films and characterize the {Con A/Dex}(n)-Mb films. The Mb in {Con A/Dex}(n)-Mb films showed a quasi-reversible CV peak pair for its heme Fe(III)/Fe(II) redox couple. and the Mb-loaded films could be used to electrocatalyze the reduction of oxygen and hydrogen peroxide. The formal potential of Mb (E-o') and the surface concentration of electroactive Mb (Gamma*) in the films were affected significantly by the environmental pH. The driving forces of {Con A/Dex}(n) film assembly and Mb loading into the films were investigated. This model protein-loaded LBL films may guide us to develop novel electrochemical biosensors based on the direct electrochemistry of enzymes. (C) 2009 Elsevier Ltd. All rights reserved.
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