Journal
ELECTROCHIMICA ACTA
Volume 54, Issue 10, Pages 2823-2827Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.electacta.2008.11.025
Keywords
Horseradish peroxidase; Three-dimensional macroporous; TiO2; Photoelectrochemical; Biosensors
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Funding
- National Natural Science Foundation of China [20676038]
- Key Project of Science and Technology for Ministry of Education [107045]
- Program of Shanghai Subject Chief Scientist [08XD1401500]
- Shanghai Leading Academic Discipline [B502]
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Three-dimensional macroporous TiO2 was synthesized by a sol-gel procedure using polystyrene colloidal crystals as templates. SEM showed that a face-centered Cubic (FCC) 3D macroporous structure was obtained. Horseradish peroxidase (HRP) was successfully immobilized on the surface of an optically transparent electrode (OTE). Photoelectrochemical properties were characterized using a three electrodes system and an ultraviolet lamp. The HRP/TiO2/OTE displays a rapid photocurrent response, approximately 178.7 nA, under UV illumination (380 nm). The sensitivity of H2O2 detection was 70.04 mu A/mM without UV illumination, and it increased to 102.97 mu A/mM when illuminated by UV. The amperometric response was also enhanced. The high response was due to the good biocompatibility of TiO2 and excellent photoelectrical property and the large effective surface of the three-dimensionally ordered macroporous structure. (C) 2008 Elsevier Ltd. All rights reserved.
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