Journal
ELECTROCHEMISTRY COMMUNICATIONS
Volume 38, Issue -, Pages 131-133Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.elecom.2013.11.022
Keywords
Cysteine; Metallo-beta-lactamase; Self-assembled monolayer (SAM); beta-Lactam antibiotic; Lineweaver-Burk plot
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Funding
- Sao Paulo State Research Funding Agency (FAPESP)
- Sao Paulo State University (UNESP)
- CNPq (Brazilian National Research Council)
- Portuguese Fundacao para a Ciencia e a Tecnologia (FCT) [SFRH/BPD/76544/2011]
- Fundação para a Ciência e a Tecnologia [SFRH/BPD/76544/2011] Funding Source: FCT
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A biosensor for penicillin G was created by immobilizing penicillinase to a gold electrode by means of a cysteine self-assembled monolayer. The biosensor amperometrically monitored the catalytic hydrolysis of penicillin in a very sensible manner. Furthermore, it was successfully used to measure the Michaelis-Menten enzymatic constant and a low limit of detection of 4.5 nM was obtained. (C) 2013 Elsevier B.V. All rights reserved.
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