Journal
ACS NANO
Volume 9, Issue 4, Pages 4475-4483Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.5b01074
Keywords
fungal infection; nanoparticles; nanofibers; viruses; peptides
Categories
Funding
- National Natural Science Foundation of China [81028010, 81373231]
- Jilin Provincial Government of the People's Republic of China [20130727034YY]
- Ministry of Science and Technology of the People's Republic of China [2014DFA31740]
- National Science Foundation [CMMI-1234957, DMR-0847758, CBET-0854465]
- National Institutes of Health [1R21EB015190]
- Department of Defense Peer Reviewed Medical Research Program [W81XWH-12-1-0384]
- Oklahoma Center for the Advancement of Science and Technology [HR14-160]
- Oklahoma Center for Adult Stem Cell Research [434003]
- Division Of Materials Research
- Direct For Mathematical & Physical Scien [847758] Funding Source: National Science Foundation
- Div Of Civil, Mechanical, & Manufact Inn
- Directorate For Engineering [1234957] Funding Source: National Science Foundation
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Candida albicans (C. albicans) infection causes high mortality rates within cancer patients. Due to the low sensitivity of the current diagnosis systems, a new sensitive detection method is needed for its diagnosis. Toward this end, here we exploited the capability of genetically displaying two functional peptides, one responsible for recognizing the biomarker for the infection (antisecreted aspartyl proteinase 2 IgG antibody) in the sera of cancer patients and another for binding magnetic nanoparticles (MNPs), on a single filamentous fd phage, a human-safe bacteria-specific virus. The resultant phage is first decorated with MNPs and then captures the biomarker from the sera. The phage-bound biomarker is then magnetically enriched and biochemically detected. This method greatly increases the sensitivity and specificity of the biomarker detection. The average detection time for each serum sample is only about 6 h, much shorter than the clinically used gold standard method, which takes about 1 week. The detection limit of our nanobiotechnological method is approximately 1.1 pg/mL, about 2 orders of magnitude lower than that of the traditional antigen-based method, opening up a new avenue to virus-based disease diagnosis.
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