Direct electrochemistry of cytochrome c on a graphene/poly (3,4-ethylenedioxythiophene) nanocomposite modified electrode

The direct electrochemistry of cytochrome c (Cyt c) embedded in a novel support matrix of graphene/poly (3,4-ethylenedioxythiophene) (G-PEDOT) nanocomposite on a glassy carbon electrode was studied. The prepared G-PEDOT nanocomposite is of good biocompatibility, enhanced electric conductivity and large specific surface area. The immobilized Cyt c in G-PEDOT matrix (graphene weight percentage<2%) displays excellent direct electrochemistry and retains its biocatalytic activity toward the reduction of hydrogen peroxide. The electrochemical signal shows a linear response to H2O2 in the concentration range from 5.0 x 10(-7) to 4.0 x 10(-4) M with a detection limit of 2.49 x 10(-7) M. The results demonstrate that the G-PEDOT nanocomposite offers a biocompatible material for the construction of biofuel cells, bioelectronics and biosensors. (c) 2012 Elsevier B.V. All rights reserved.