Direct electron transfer of bilirubin oxidase (Myrothecium verrucaria) at an unmodified nanoporous gold biocathode

Well defined mediatorless bioelectrocatalytic reduction of oxygen with high current densities of 0.8 mA cm(-2) was obtained on nanoporous gold electrodes modified with Myrothecium verrucaria bilirubin oxidase. A stable faradaic response was observed when the enzyme modified electrode was coated with a specifically designed electrodeposition polymer layer. The response of the enzyme electrode was only slightly inhibited by the addition of F-. (C) 2011 Elsevier B.V. All rights reserved.