Journal
ELECTROCHEMISTRY COMMUNICATIONS
Volume 22, Issue -, Pages 137-140Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.elecom.2012.06.019
Keywords
Electrochemical DNA sensor; Hybridization; Gold electrode; Osmium tetroxide bipyridine; Real sample; Maize MON810
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Funding
- German Research Foundation (DFG) [FL 384/11-1, FL 384/7-1]
- Federal Ministry for Education and Research (BMBF)
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We demonstrate an approach for the electrochemical detection of genetically modified maize in real maize flour samples by means of square-wave voltammetry (SWV). After labeling the asymmetric PCR-amplified targets with osmium tetroxide bipyridine [OsO4(biPY)], they were hybridized with immobilized oligonucleotide probes on gold electrodes. We could detect the maize genes ivrp and SSIIb in near isogenic maize. The transgene cryla/b and the MON810 specific fragment were detected in all transgenic maize samples, down to a content of 0.6% of MON810 in mixed samples. While it was possible to detect all sequences in the samples containing 100% near isogenic or respectively transgenic maize after a hybridization time of less than 10 min, a hybridization time of 30 min was necessary for the detection of the genetic modifications in samples containing only 0.9 to 0.6% of transgenic maize. No significant detection of the transgene cryla/b or MON810 was possible when only 0.5% of transgenic maize was present in the sample, most likely due to insufficient amplification of the template DNA. (C) 2012 Elsevier B.V. All rights reserved.
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