Journal
ELECTROCHEMISTRY COMMUNICATIONS
Volume 12, Issue 1, Pages 175-177Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.elecom.2009.11.020
Keywords
Graphene; Chitosan; Cytochrome c; Biosensor
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Funding
- National Nature Science Foundation of China [20975001]
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Chitosan-dispersed graphene nano-flakes were prepared by a chemical route to reduce graphene oxide and dispersed fully in water to form a stable black aqueous solution. The as-prepared graphene nano-flakes were successfully immobilized on glassy carbon electrode to construct a graphene modified electrode. Cytochrome c was adsorbed tightly on the surface of the modified electrode and the direct electron transfer of Cytochrome c was achieved. Cytochrome c on the surface of electrode maintains its bioactivity and shows an enzyme-like activity for the reduction of nitric oxide, displaying a potential application for the fabrication of novel biosensors to sense nitric oxide. This research will enlarge the applications of graphene-based materials in biosensor field. (C) 2009 Elsevier B.V. All rights reserved.
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