Journal
ELECTROCHEMISTRY COMMUNICATIONS
Volume 12, Issue 3, Pages 471-474Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.elecom.2010.01.022
Keywords
Prostate-specific antigen (PSA); Electrochemistry; Peptide; Ferrocene
Categories
Funding
- North Dakota Experimental Program to Stimulate Competitive Research (EPSCoR)
- North Dakota State University
- Pacific Northwest National laboratory (PNNL)
Ask authors/readers for more resources
This paper presents a novel approach to electrochemically determine enzymatically active PSA using ferrocene-functionalized helix peptide (CHSSLKQK). The principle of electrochemical measurement is based on the specific proteolytic cleavage events of the FC-peptide on the gold electrode surface in the presence of PSA, resulting the change of the current signal of the electrode. The percentage of the decreased current is linear with the concentration of active PSA at the range of 0.5-40 ng mL(-1) with a detection limit of 0.2 ng mL(-1). The direct transduction of peptide cleavage events into an electrical signal provides a simple, sensitive method for detecting the enzymatic activity of PSA and determining the active PSA concentration. Published by Elsevier B.V.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available