Journal
ELECTROCHEMISTRY COMMUNICATIONS
Volume 11, Issue 5, Pages 1009-1012Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.elecom.2009.02.044
Keywords
Electrochemical immunosensor; Cancer biomarker; Carbon nanotubes; Gold nanoparticles; Protein detection
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Funding
- National Center for Research Resource (NCRR) [P20RR016457]
- NIEHS/NIH [ES013557]
- National Institute of Dental and Craniofacial Research, NIH
- Science Foundation Ireland
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Electrochemical immunosensors based on single wall nanotube (SWNT) forests and 5 nm glutathione-protected gold nanoparticles (GSH-AuNP) were developed and compared for the measurement of human cancer biomarker interleukin-6 (IL-6) in serum. Detection was based on sandwich immunoassays using multiple (14-16) horseradish peroxidase labels conjugated to a secondary antibody. Performance was optimized by effective blocking of non-specific binding (NSB) of the labels using bovine serum albumin. The GSH-AuNP immunosensor gave a detection limit (DL) of 10 pg mL(-1) IL-6 (500 amol mL(-1)) in 10 mu L. calf serum, which was 3-fold better than 30 pg mL(-1) found for the SWNT forest immunosensor for the same assay protocol. The GSH-AuNPs platform also gave a Much larger linear dynamic range (20-4000 pg mL(-1)) than the SWNT system (40-150 pg mL(-1)), but the SWNTs had 2-fold better sensitivity in the low pg mL(-1) range. (C) 2009 Elsevier B.V. All rights reserved.
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