A sensitivity-controlled hydrogen peroxide sensor based on self-assembled Prussian Blue modified electrode

In this communication, a hydrogen peroxide (H2O2) sensor based on self-assembled Prussian Blue (PB) modified electrode was reported. Thin film of PB was deposited on the electrode by self-assembly process including multiple sequential adsorption of ferric ions and hexacyanoferrate ions. The as-prepared PB modified electrode displayed sufficient stability for practical sensing application. At an applied potential of -0.05 V vs. Ag/AgCl (sat. KCI), PB modified electrode with 30 layers exhibited a linear dependence on H2O2 concentration in the range of 1 X 10(-6)-4 x 10(-4) M (r = 0.9998) with a sensitivity of 625 mA M-1 cm(-2). It was found that the sensitivity of H2O2 sensors could be well controlled by adjusting the number of deposition cycles for PB preparation. This work demonstrates the feasibility of self-assembled PB modified electrode in sensing application, and provides an effective approach to control the sensitivity of PB-based amperometric biosensors. (C) 2008 Elsevier B.V. All rights reserved.