Journal
ELECTROCHEMISTRY COMMUNICATIONS
Volume 11, Issue 10, Pages 2048-2051Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.elecom.2009.08.048
Keywords
Glucose; Nanostructured Au; Glucose oxidase; Biosensor; Prussian blue
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Funding
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Canada Foundation of Innovation (CFI)
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We report on a novel glucose biosensor based on the immobilization of glucose oxidase (GOx) on a Prussian blue modified nanoporous gold surface. The amperometric glucose biosensor fabricated in this study exhibits a fast response and the very low detection limit of 2.5 mu M glucose. The sensitivity of the biosensor was found to be very high, 177 mu A/mM; the apparent Michaelis-Menten constant is calculated to be 2.1 mM. In addition, the biosensor has good reproducibility and remains stable over 60 days. The anti-interference ability of the biosensor was also assessed, showing little interference from possible interferents such as ascorbic acid (AA), acetaminophen (AP) and uric acid (UA). (C) 2009 Elsevier B.V. All rights reserved.
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