Journal
ELECTROCHEMISTRY COMMUNICATIONS
Volume 11, Issue 4, Pages 783-786Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.elecom.2009.01.035
Keywords
TiO2 nanocubes; PABSA nanofibres; DNA damage; Co(phen)(3)(3+)
Categories
Funding
- National Natural Science Foundation of China [20635020, 20805025]
- Doctoral Foundation of the Ministry of Education of China [20060426001]
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A novel membrane comprising of hollow TiO2 nanocubes and poly(m-aminobenzene sulfonic acid) (PABSA) nanofibres was constructed on the glassy carbon electrode (GCE) for detecting the oxidative damage of natural dsDNA induced by TiO2 under UV radiation. The TiO2/PABSA membrane was used for efficient dsDNA immobilization, dsDNA oxidation through photogenerated hydroxyl radicals, and as the platform for electrochemical sensing. The properties of the TiO2/PABSA membrane and the immobilization of dsDNA were characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy using [Fe(CN)(6)](3-/4-) as the indicator. The resulting oxidative damage of dsDNA was detected by monitoring the CV response of an intercalated electroactive probe, namely, Co(phen)(3)(3+) PABSA has a profound synergistic effect with the hollow TiO2 on the DNA damage detection and the sensor has the potential of becoming a powerful tool for the rapid assessment of the genotoxicity of existing and new chemicals. (C) 2009 Published by Elsevier B.V.
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