Journal
ELECTROCHEMISTRY COMMUNICATIONS
Volume 11, Issue 2, Pages 359-362Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.elecom.2008.11.049
Keywords
Peptide nucleic acid end-labeling; Osmium tetroxide complexes; Electroactive labels; Adsorptive stripping voltammetry; Mercury and carbon electrodes; Electrochemical DNA sensors
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Funding
- Academy of Sciences of the Czech Republic [KAN400310651]
- Ministry of Education, Youth and Sports of the CR [LC06035]
- [AV0Z50040507]
- [AV0Z50040702]
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Peptide nucleic acid (PNA), the DNA mimic with electrically neutral pseudopeptide backbone, is intensively used in biotechnologies and particularly in single-base mismatch detection in DNA hybridization sensors. We propose a simple method of covalent end-labeling of PNA with osmium tetroxide, 2,2'-bipyridine (Os,bipy). Os,bipy-modified PNA (PNA-Os,bipy) produces voltammetric stripping peaks at carbon and mercury electrodes. Peak potential (E(p)) of one of the anodic peaks of PNA-Os,bipy at the pyrolytic graphite electrode (PGE) differs from E(p) of the reagent, allowing PNA-Os,bipy analysis directly in the reaction mixture. At the hanging mercury drop electrode (HMDE) the PNA-Os,bipy yields a catalytic peak Cat(p) in addition to the redox couples. Using Cat(p) it is possible to detect purified PNA-Os,bipy down to 1 pM concentration at accumulation time 60 s. To our knowledge this is the highest sensitivity of the electrochemical detection of PNA. (C) 2008 Elsevier B.V. All rights reserved.
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