Multilayer structured carbon nanotubes/poly-L-lysine/laccase composite cathode for glucose/O2 biofuel cell

Multilayer film of laccase, poly-L-lysine (PLL) and multi-walled carbon nanotubes (MWNTs) were prepared by a layer-by-layer self-assembly technique. The results of the UV-vis spectroscopy and scanning electron microscopy studies demonstrated a uniform growth of the multilayer. The catalytic behavior of the modified electrode was investigated. The (MWNTs/PLL/laccase)(n) multilayer modified electrode catalyzed four-electron reduction of O-2 to water, without any mediator. The possible application of the laccase-catalyzed O-2 reduction at the (MWNTs/PLL/laccase)(n) multilayer modified ITO electrode was illustrated by constructing a glucose/O-2 biofuel cell with the (MWNTs/thionine/AuNPs)(8) GDH film modified ITO electrode as a bioanode and the (MWNTs/PLL/laccase)(15) film modified ITO electrode as a biocathode. The open-circuit voltage reached to 700 mV, and the maximum power density achieved 329 mu W cm(-2) at 470 mV of the cell voltage. (c) 2008 Elsevier B.V. All rights reserved.