Journal
ELECTROCHEMISTRY COMMUNICATIONS
Volume 10, Issue 9, Pages 1246-1249Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.elecom.2008.06.003
Keywords
sol-gel; cerium oxide; nanoporous; cholesterol oxidase; electrochemical biosensor
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Funding
- National Physical Laboratory, New Delhi, India
- CSIR, India
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Cholesterol oxidase (ChOx) has been immobilized onto sol-gel derived nano-structured cerium oxide (NS-CeO2) film deposited on indium-tin-oxide (ITO) coated glass substrate. Phase identification of sol-gel NS-CeO2 film carried out using X-ray diffraction (XRD) yields reflection peak at 29.4 degrees corresponding to (111) plane with oriented crystallite (34 nm) along c-axis normal to the substrate. Electrochemical studies reveal that NS-CeO2 provides electroactive surface for the loading of ChOx and enhances electron transfer rate in the ChOx/NS-CeO2/ITO bioelectrocle. The low value of Michaelis-Menten constant (K-m) obtained as 2.08 mM indicates enhanced ChOx affinity to cholesterol. The observed results show application of sol-gel derived NS-CeO2 for biosensing without any functionalization. (C) 2008 Elsevier B.V. All rights reserved.
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