Journal
ELECTROCHEMISTRY COMMUNICATIONS
Volume 10, Issue 1, Pages 38-41Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.elecom.2007.10.030
Keywords
cytochrome c; room temperature ionic liquid; gold nanoparticles; multi-walled carbon nanotubes; direct electron transfer
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A robust and effective composite film based on gold natioparticles (GNPs)/room temperature ionic liquid (RTIL)/multi-wall carbon nanotubes (MWNTs) modified glassy carbon (GC) electrode was prepared by a layer-by-layer self-assembly technique. Cytochrome c (Cyt c) was successfully immobilized on the RTIL-nanohybrid film modified GC electrode by electrostatic adsorption. Direct electro-chemistry and electrocatalysis of Cyt c were investigated. The results suggested that Cyt c could be tightly adsorbed on the modified electrode. A pair of well-defined quasi-reversible redox peaks of Cyt c was obtained in 0.10 M, pH 7.0 phosphate buffer solution (PBS). RTIL-nanohybrid film showed an obvious promotion for the direct electron transfer between Cyt c and the underlying electrode. The immobilized Cyt c exhibited an excellent electrocatalytic activity towards the reduction of H2O2. The catalysis currents increased linearly to the H2O2 concentration in a wide range of 5.0 x 10(-5) - 1.15 x 10(-3) M. Based on the multilayer film, the third-generation biosensor could be constructed for the determination of H2O2. (C) 2007 Elsevier B.V. All rights reserved.
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