Direct electrochemistry of hemoglobin and its electrocatalytic effect based on its direct immobilization on carbon ionic liquid electrode

Direct electrochemistry of hemoglobin (Hb) has been achieved by its direct immobilization on carbon ionic liquid electrode (CILE). CILE was immersed in a solution containing Hb and ionic liquid, octylpyridinium chloride ([OcPy][Cl]), to directly immobilize Hb on CILE. Cyclic voltammetry of modified electrode exhibited quasi-reversible peaks corresponding to Hb. The oxidation and reduction peak potentials of immobilized Hb in acetate buffer solution, pH 5.0 and at a scan rate of 0.1 V s(-1) were obtained at about -150 mV and -290 mV, respectively. The average surface coverage of the electroactive Hb adsorbed on the electrode surface was calculated as 8.4 x 10(-11) mol cm(-2). Hb retained its bioactivity on modified electrode and showed excellent electrocatalytic activity towards oxygen, hydrogen peroxide and nitrite. Hydrogen peroxide can be determined in the range of 1.0 x 10(-4)-5.0 x 10(-3) M. (C) 2008 Elsevier B.V. All rights reserved.