Journal
ELECTROCHEMISTRY COMMUNICATIONS
Volume 10, Issue 4, Pages 630-634Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.elecom.2008.01.020
Keywords
horseradish peroxidase; nanotubes; layer-by-layer assembly; anodic aluminum oxide; direct electrochemistry
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A novel method based on electric field directed layer-by-layer assembly (EFDLA) and template synthesis is successfully developed to fabricate horseradish peroxidase (HRP) nanotubes. It provides a rapid and general strategy for functional protein nanoarrays. The alternative deposition of poly(diallyldimethylammonium chloride) (PDDA) and HRP are characterized by SEM, TEM, UV-vis and electrochemical impedance spectroscopy (EIS). Moreover, the UV-vis spectrometry and electrochemistry of HRP indicate that immobilization of PDDA/HRP via EFDLA coupled with template synthesis could retain the conformations and bioactivities of HRP. (C) 2008 Elsevier B.V. All rights reserved.
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