Journal
ELECTROCHEMISTRY COMMUNICATIONS
Volume 10, Issue 10, Pages 1502-1504Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.elecom.2008.07.051
Keywords
Telomerase; PCR-free; Guanine; Oxidation signal; Differential pulse voltammetry
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Funding
- National Natural Science Foundation of China [90406005, 20575028]
- Program for New Century Excellent Talents in University
- Chinese Ministry of Education [NCET-04-0452]
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In this paper, we report a non-PCR-based electrochemical assay that can detect telomerase activity. Telomerase from HeLa cells may induce telomerization of thiolated primers immobilized on a gold electrode surface. With the telomerization reaction, more and more guanine-rich telomeric repeats are formed, so the electrochemical oxidation signal of guanine at about 1.00 V, which is utilized to indicate the elongated guanine-rich telomeric repeats tethered to the primers, will be increased. This assay method can detect the telomerase activity originated from 3000 HeLa cells and thus holds promise as a simple and sensitive approach in clinical diagnosis of cancer. (c) 2008 Elsevier B.V. All rights reserved.
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