Journal
ELECTROANALYSIS
Volume 26, Issue 11, Pages 2465-2473Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.201400353
Keywords
Hemoglobin; Cadmium sulphide; Quantum dots; Chitosan; Hydrogen peroxide
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Funding
- Science Foundation Ireland (SFI) for the Irish Separation Science Cluster (ISSC) Grant [08/SRC/B1412]
- Walton Visitor Award
- Thailand Research Fund (TRF)
- Rajamangala University of Technology Isan (RMUTI) through the TRF [TRG5780076]
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An L-cysteine capped cadmium sulfide-chitosan nanocomposite has been synthesized, characterized and used for surface modification of a glassy carbon electrode. With direct electron transfer, hemoglobin (Hb) adsorbed strongly on the nanocomposite and displayed excellent bioelectrocatalysis for H2O2. The biosensor was capable of reducing H2O2 at -0.35 V, with a detection limit of 3.13 nM, linearity in the range of 15 nM to 10 mu M and a response time of less than 2 s. The Michaelis-Menten constant (K-M) was 0.57 nM, attesting high bioelectrocatalysis of immobilized Hb for H2O2. Reproducibility of the fabrication method was very satisfactory with a relative standard deviation of 5.3%. The biosensor lost only 6.5% of its original response after 7 days when stored in a pH 7.4 PBS at 4 degrees C.
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