Journal
ELECTROANALYSIS
Volume 24, Issue 7, Pages 1547-1553Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.201200111
Keywords
Enzyme biosensors; Cholesterol oxidase; Carbon film electrodes; Poly(neutral red); Egg yolk
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Funding
- Fundacao para a Ciencia e a Tecnologia (FCT), Portugal [PTDC/QUI/65732/2006, PTDC/QUI-QUI/116091/2009]
- POCI
- COMPETE
- European Community
- CEMUC (Research Unit 285), Portugal
- FCT [SFRH/BPD/36930/2007]
- Fundação para a Ciência e a Tecnologia [SFRH/BPD/36930/2007, PTDC/QUI-QUI/116091/2009, PTDC/QUI/65732/2006] Funding Source: FCT
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New amperometric cholesterol oxidase (ChOx) based enzyme biosensors for cholesterol have been developed. The enzyme was immobilised with and without glutaraldehyde cross-linking on top of carbon film electrodes modified with redox mediators. Mediators tested were: poly(neutral red) (PNR), Prussian blue and cobalt hexacyanoferrates. Amperometric detection of cholesterol showed that PNR/ChOx modified electrodes exhibited the best characteristics; under optimised conditions cholesterol was determined at -0.4 V vs. SCE with a detection limit of 1.9 mu M. The biosensors showed good reproducibility and stability and only a small influence from potential interferents in food. Analyses of cholesterol in egg yolk were successfully performed.
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