Journal
ELECTROANALYSIS
Volume 24, Issue 5, Pages 1057-1064Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.201100734
Keywords
Aptamer; Binding affinity; Electrochemical method; Dissociation constant; Microarray chip
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Funding
- National Research Fund
- Ministry of Education, Science and Technology, South Korea [20100029128]
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Simultaneous evaluation of the binding affinity of a series of aptamers toward a target molecule was investigated using an electrochemical microarray chip. The chip was modified by immobilizing seven aptamers obtained from the SELEX process and a control sequence onto gold nanoparticle-comprised conducting polymer-coated microarray electrodes. The chip was then incubated with the target molecule, kanamycin. The electrochemical response of the captured kanamycin was studied to evaluate the binding affinity of the aptamers, which showed the same trend with the fluorescence spectroscopic results. The lowest dissociation constant between a selected aptamer and kanamycin was determined to be 38.06 +/- 0.73 nM.
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