Journal
ELECTROANALYSIS
Volume 24, Issue 12, Pages 2283-2290Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.201200403
Keywords
TiO2 nanorods; Graphene; Chitosan; Carbon ionic liquid electrode; Electrochemical DNA biosensor
Categories
Funding
- Public Service Sectors (Agriculture) Research Projects of the Ministry of Science and Technology [200903055]
- Research Project of the General Administration of Quality Supervision, Inspection and Quarantine of P.R. China [2012IK175]
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Based on graphene (GR), TiO2 nanorods, and chitosan (CTS) nanocomposite modified carbon ionic liquid electrode (CILE) as substrate electrode, a new electrochemical DNA biosensor was effectively fabricated for the detection of the transgenic soybean sequence of MON89788. By using methylene blue (MB) as hybridization indicator for monitoring the hybridization with different ssDNA sequences, the differential pulse voltammetric response of MB on DNA modified electrodes were recorded and compared. Due to the synergistic effects of TiO2 nanorods and GR on the electrode surface, the electrochemical responses of MB were greatly increased. Under optimal conditions the differential pulse voltammetric response of the target ssDNA sequence could be detected in the range from 1.0x10-12 to 1.0x10-6 mol/L with a detection limit of 7.21x10-13 mol/L (3s). This electrochemical DNA biosensor was further applied to the polymerase chain reaction (PCR) product of transgenic soybeans with satisfactory results.
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